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An AquaNew® Water

All products, written information, testimonials, and other statements provided on this website have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure, or prevent any disease. For all health concerns, please consult with an appropriate licensed healthcare practitioner.

STUDIES

Watt-Ahh®, Ultra-pure Polarized Water, is produced from a new Technology licensed by LSG Partners, L.L.C. Our mutual mission is to continue to perform clinical studies to quantify the benefits of Watt-Ahh® and SG Gas or hydroxyhydrogen gas that is infused into ultra-pure water to create Watt-Ahh® (also identified herein as SG Gas-infused Water or Ultra-pure Polarized Water). The following is a summary of studies completed to date and we recommend that you check back on a regular basis as we will continue to post the results of additional studies and clinical trend trials. For more information on the science behind Watt-Ahh®, please visit the website for our R&D Company, WIT International, L.L.C., www.WaterIonTechnologies.com.

Quality of Watt-Ahh®, Bottled Drinking Water
We use quality control practices in producing Watt-Ahh® at a licensed water plant and guarantee 100% customer satisfaction on our products. The following information is our best available characterization of product quality based on random samples for testing.

Water Purity (Quality)

a. Protocol: Samples of bottled Watt-Ahh® produced on January 17, 2010 were sent to the lab for analyses.

Certified Lab: A nationally-recognized lab located in San Antonio, Texas

Test Results: The parameters regulated by the U.S. Food and Drug Administration (FDA) were tested and the results on this link show Watt-Ahh® samples either were not detected (below thresholds of detection by the lab) or sub-trace contaminants that far exceed the FDA regulations. For a copy of the full report, please send an email request to inquiries@aquanew.com.

b. Protocol: Random samples of bottled Watt-Ahh® were taken to the lab for analyses.
Certified Lab: Sanders Laboratories, Inc., Nokomis, FL

Test Results:

Calcium Hardness	At or below MDL (Method Detection Level of 3 mg/l CACO3)
Hardness, Total	At or below MDL (MDL is 3 mg/l CACO3)
Specific Conductivity	6.2 umhos/cm
Total Dissolved Solids	At or below MDL (MDL is 20 mg/l)
Turbidity	At or below MDL (MDL is 0.1 NTU or Nephelometric Turbidity Unit)

Hydroxyhydrogen Gas Purity (Non-toxic)
Protocol: Samples of hydroxyhydrogen gas were used for a Vero Cell Cytotoxicity Assay (SOP #C-19B). The day before the start of the assay, a 96 well plate was seeded with the appropriate number of viable Vero cells. The next appropriate dilutions of the positive and negative controls (Shiga Toxin STX 1) were prepared in tissue culture medium as well as dilutions of the samples. The medium was aseptically removed from the Vero cells and it was replaced with the various control and test solutions. The plate was then incubated approximately 48 hrs. in a 37°C tissue culture incubator. After incubation, the plate was further processed according to the Standard Operating Procedures. A tetrazolium dye, MTT, is added to the wells of the plates. The plates are incubated a minimum of 4 hrs. in the presence of the dye. The yellow dye is taken up and converted to a blue color only by living cells. The plates are then read on an ELISA plate reader and the data from the samples are compared to the data from the controls.

Certified Lab: Toxin Technology, Inc., Sarasota, FL.

Test Results: All samples are negative with respect to cytotoxic activity with respect to the controls in the assay (sensitivity approximately 1 ng STX 1/ml) at the dilutions tested (1:10, 1:20, 1:40 and 1:80 dilutions). (Toxin Technology, Inc., report dated 2006).
Capacitance (Smaller clusters of water molecules for improved hydration and more efficient metabolism)

Protocol: A gallon of pure water is infused with SG Gas using a bubbling tube for a period up to 20 minutes. Total Dissolved Solids (TDS) is measured in the water prior to and subsequent to bubbling. A Control Company Traceable™ #4063CC meter is used to measure TDS changes between untreated and treated (SG Gas infused) water. A Fluke 189 True RMS Multimeter is used to measure drop in capacitance in the water during bubbling with SG Gas. The factors affecting the absorption rate of SG Gas include initial purity of water, size of gas bubbles and volume of water to be treated.

Testing Location: Performed at WIT corporate workshop, Sarasota, FL.

Test Results: Absorption over time or saturation graphs show water treated with SG Gas has a lower capacitance level which indicates less electrical resistance or conductivity among smaller clusters of water molecules.

Untreated Water (not infused with SG Gas) – 0.33 ppm TDS; 24 nF Capacitance
Treated Water (infused with SG Gas) – 0.17 ppm TDS after 11 minutes of bubbling with SG Gas; 10 nF Capacitance (a net decrease of 13 nF when compared to that of Untreated Water).
Notes: nF (nano farads) is one-one thousand of a uF (micro farads). ppm is the abbreviation for parts per million.
Redox (Reduction/Oxidation) Potential or ORP

Background: For fruit and vegetable production, microbial quality of water used in harvesting, cooling, transporting, postharvest drenching and other procedures is critical in eliminating waterborne microorganisms including postharvest plant pathogens and agents of human illness. Many packers of fresh produce use sensors to monitor the oxidation-reduction potential (ORP) status of their water systems. According to Division of Agriculture and Natural Resource of the University of California*, “…ORP, measured in millivolts (mV) has increasingly become a primary approach to standardizing water disinfection parameters…since ORP reflects the antimicrobial potential of water…” From the UofC report referenced below, E. coli dies in less than 60 seconds and Salmonella spp. dies in over 300 seconds within a solution likely with the addition of sodium hypochlorite (bleach) or chlorine, having an ORP reading between 550 and 620 mV. A relatively high ORP of Watt-Ahh® provides good cleaning power without extensive use of harsh chemicals including chlorine which is prohibited in Europe. We find cleaning properties are maintained when one part of commercial cleaning solution is diluted with 20 parts of Watt-Ahh®. As an alternative, oxygen is a more powerful oxidizer when compared to that of chlorine. An ORP of 500 using an oxygen-based oxidizer is more effective than an ORP of 750 using a chlorine-based oxidizer. Chlorine is typically used in municipal systems since it is less expensive. If organisms can not be killed with chlorine, the municipality will likely revert to either ozone or peroxide treatment that is more effective. Due to the ionized nature of our molecule, we are of the opinion that it is more effective when compared to that of peroxide. Watt-Ahh® has an ORP greater than 370 mV based on the test results below which indicate weak to moderate antibacterial properties. The effective elimination of free-floating microbes on the surfaces of fruits and vegetables, kitchen counters and bathrooms could occur through the use of either Watt-Ahh® or SG Gas as an oxidizer, which may very well avoid the need to add a supplemental disinfectant treatment such as chlorine, ozone, peroxide or UV irradiation.

* “Oxidation-Reduction Potential (ORP) for Water Disinfection Monitoring, Control, and Documentation”, Trevor V. Suslow, Extension Research Specialist, Department of Vegetable Crops, University of California, Davis, Publication 8149, ISBN 978-1-60107-319-8, 4 pp., 2004.

Protocol: Random samples of bottled Watt-Ahh® were taken to the lab for ORP analyses.

Certified Lab: Xenco Laboratories, Tampa, FL.

Test Results: Testing performed by Xenco Laboratories revealed a positive ORP reading for Watt-Ahh®, which indicates Ultra-pure Polarized Water will serve to prevent bacterial growth. The results follow:

Watt-Ahh® Redox Potential Between 483 and 516 mV
(Xenco Laboratories, report dated September 2008).

Watt-Ahh® Redox Potential Between 460 and 462 mV on water samples produced on January 17,2010.
(Xenco Laboratories, report dated March 2010).

Watt-Ahh® Redox Potential Between 379 and 380 mV on water samples produced on January 17, 2010
(A nationally-recognized laboratory located in San Antonio, Texas, report dated June 22, 2010. For a copy of the full report, please send an email request to inquiries@aquanew.com).

Watt-Ahh® Redox Potential Between 454 and 455 mV on water samples produced on January 17, 2010
(Xenco Laboratories, report dated July 13, 2010).
pH

Background: Based on our scientific literature search, ultra-pure water has a pH between 6.8 and 7.4. The pH of ultra-pure water bubbled with SG Gas can not be accurately determined using litmus paper or other conventional testing methods for pH due to free hydrogen in the water.
Water Storage (binding of SG Gas into water)

Observations: Polarized Water produced over 4 years ago and contained in a clear plastic spray bottle, with mostly daily exposure to the Florida sun remains clear in appearance with no evidence of either mold or scale on the interior surface of the container. Governing laws require expiration dates of 2 years on each water bottle produced; however, we are of the opinion that Ultra-pure Polarized Water with SG Gas will remain permanently stable in a container. We can demonstrate that SG Gas remains bonded to water molecules by freezing Polarized Water into ice cubes. SG Gas does not freeze at 32o F. Instead, gas bubbles form within the ice cube itself, sometimes producing on the surface of the ice cubes, capillary tubes where the SG Gas escapes.

Other Uses for Watt-Ahh® – Plant Growth and Long Shelf-life for Cut Flowers
1. Cut Flowers
  
  Protocol: In a residential-setting with a room temperature between 74 and 76^o F. and AC house humidity, two similar clean glass vases were used such that one vase was filled with Ultra-pure Polarized Water (or SG Gas-infused Water or “SG Water”) and the other was filled with municipal tap water from San Antonio, Texas. Two sets of roses were clipped and immersed in their respective waters two times and then set in place next to each other. Photos were taken for five consecutive days.
  
  Test Location: Eugene Dedick, has a Masters in P-Chem and a water consultant performed this test in San Antonio, Texas.
  
  Test Results: Mr. Dedick reports the cut roses in a vase filled with Ultra-Pure Polarized Water outlasted roses in tap water by 20% to 30% longer. Mr. Dedick writes about the following observations… “This increased shelf life for the Polarized Water flowers is substantial for $60 per dozen roses and the logistic/shipping savings for holdovers and reduced wastage is significant. The tap water flowers wilted, bent over, and became flaccid two days before any flowers in the Polarized Water started to bend. The Polarized Water flowers opened up and stayed turgid after they started to ‘bow’. Also, the Polarized Water had a ‘flower smell’ and the tap water had a moldy smell like the bugs were taking over. Additionally, it is interesting to note that on some of the flowers in Polarized Water, there was actually tan water dripping out of the rose heads. It was as if their xylem was still pumping water at a living rate to the rose heads. I have never seen this before. It’s like the roses in Polarized Water want to ‘live’ after they had been thru the guillotine”. (Eugene Dedick - Masters in P-Chem, report dated July 15, 2010).
2. Ivy Plants
  
  Protocol: In a controlled greenhouse setting, four groups of ivy plants were watered using (1) 100% well water, (2) mix of 1/3 mix SG Gas-infused Water and 2/3 well water, (3) mix of 2/3 SG Gas-infused Water and 1/3 well water, and (4) 100% SG Gas-infused Water. The ivy plants were harvested and dehydrated to allow measurement of dry plant mass.
  
  Test Location: Commercial greenhouse grower, Ramona Reiser of R&R Expectations, Sarasota, FL performed this study.
  
  Test Results: The fourth group of 100% SG Gas-infused Water has over 16 percent increase in mass when compared to that of the first group of well water. (Ramona Reiser, report dated 2006)

Other Uses for Watt-Ahh® – Nutrient Absorption

Vitamin C

Protocol: In a controlled experiment, a standard drug metabolism test in vitro was conducted over a period of 21 days. This comparative test was performed on cell membrane permeability for Vitamin C solution (L-ascorbic acid) using (1) Hank’s Buffered Saline Solution (HBSS) and (2) SG Gas-infused Water. Caco-2 cells were used and permeability of the apical side (similar to intestine surface) and basolateral side (similar to underneath intestinal surface) for the separate solutions were determined. Vitamin C quantification was conducted on HPLC (HP1100 equipped with PDA detector) and Zorbax C18 reverse phase column (4.6 x 250 mm, 5 micro) at 30C.
Certified Lab: University of British Columbia, Canada

Test Results: Vitamin C permeability of SG Gas-infused Water is approximately 4 times higher than the control counterpart. (Charles Hu, communication in 2008)

Wheatgrass

Protocol: Wheatgrass seeds were soaked for 72 hours in a bowl filled with Watt-Ahh™. Simultaneously, Grodan plugs were soaked in a bowl filled with Watt-Ahh™ and a couple of teaspoons of baking soda. The wet Grodan plugs (growth medium) are squeezed to remove excess water and placed in small plastic webbed containers that fit holes in an Aerogarden Kitchen Aeroponic Garden system. Growth medium and plastic container are carefully placed in each hole adjacent to the water jet inside the water tray. The germinated Wheatgrass seeds are placed on top of the growth medium. Small clear plastic cups are placed on top of each growth medium to maintain ambient moisture surrounding the seedlings. The water tray is filled with Watt-Ahh™ to the specified level inside the tray. Soluble fertilizer is added to the water in the tray. The Aerogarden is set to the Herb setting that controls the frequency and length of light exposure each day. Once two sets of leaves appear (approximately 2 to 3 days), each clear plastic cup is removed. After 10 days of growth, Wheatgrass leaves are harvested and placed in a sealed plastic bag. The sealed bag is placed inside an insulated box with a frozen plastic ice holder and immediately shipped to the lab for ORAC (Oxygen Radical Absorbance Capacity) analysis.

The ORAC analysis provides a measure of the scavenging capacity of antioxidants against the peroxyl radical, which is one of the most common reactive oxygen species (ROS) found in the body. Trolox, a water-soluble Vitamin E analog, is used as the calibration standard.

Certified Lab: Brunswick Laboratories, Norton, MA.

Test Results:

ORAC_hydro	16 umoleTE/gram	16,000 umoleTE/liter
ORAC_Lipo	3 umoleTE/gram	3,000 umoleTE/liter
ORAC_total	19 umoleTE/gram	19,000 umoleTE/liter

Notes: ORAChydro reflects water-soluble antioxidant capacity. ORACLipo is the lipid soluble antioxidant capacity. ORACtotal is the sum of ORAChydro and ORACLipo. (Brunswick Laboratories, report dated June 2006)

Other Uses for Watt-Ahh® – Ingredient in Product Improvement
1. Longer Shelf Life
  
  Protocol: Watt-Ahh™ replaces municipal water as one of the ingredients to prepare the batter for a commercial cookie enriched with high quality salmon oil. A shelf-life study is performed for at least 21 days.
  
  Test Location: Venice, FL
  
  Test Results: Mold does not appear on the cookies made with Watt-Ahh™. The chef observed Watt-Ahh™ emulsifies the salmon oil better than regular (non-polarized) water. Additionally, the taste of the cookies with Watt-Ahh™ also has a more robust flavor.
2. Moisturizing Formulas (refer to Section E.1 below).
3. Emulsification of Oils (refer to Sections D.1 and E.1 herein).
Other Uses for Watt-Ahh® – Skin Hydration and Repair
1. Lotion and Creams
  
  Protocol: Topical applications were applied twice a day on each side of the face and in vicinity of the eyes. Two types of topical solutions were prepared with 1% magnesium ascorbyl phosphate (MAP), one using SG Gas-infused Water and the other using tap water. This routine was done for 21 days.
  
  Certified Lab: Optima Specialty Chemical, Huntington, CT
  
  Test Results: On the side of the face where SG Gas-infused Water solution was applied, a slight reduction in the depth of fine lines around the eye and a lighting of darker skin pigment are observed when compared to that of the other side of the face where the tap water solution was applied. (Stephen Puleo of Optima Specialty Chemical, communication in 2008)
2. Home Test: Fill bowl with Watt-Ahh™ and place hand in the Water for over 20 minutes. Fingers remain plumb and with limited wrinkling. Regular (non-polarized) water will cause the skin to wrinkle when exposed to water for over 20 minutes which is an indication that regular water is dehydrating the skin.
3. Burns and Pain Relief (protocol has been prepared and clinical trials will commence in the near future)
Other Uses for Watt-Ahh® – Improved Cleaning (also refer to Section A.4 above)
1. Laundry
  
  Protocol: Add 1/3 gallon of Watt-Ahh™ in a standard washing machine tub of 12 gallons for medium load to soap cycle. The remaining Watt-Ahh™ (2/3 gallon) is added to the rinse cycle. The washed clothes are removed from the washer and placed into a dryer until dry. Another medium load of clothes are washed in the same washing machine and using the same detergent (no Ultra-pure Water Polarized is added to either the soap or rinse cycles).
  
  Testing Location: Performed at WIT corporate workshop, Sarasota, FL.
  
  Test Results:
  Treated Water (infused with SG Gas) – Clothes smell fresher and less body oil and odor smell during work outs. Less soap residue is evident after re-saturating dry clothes with water. The addition of Ultra-pure Polarized Water to the rinse cycle removes soap residue that may contain residual dirt from the fabric. This wash procedure using Ultra-pure Polarized Water is especially preferred for people with allergic sensitivities to residual soap in their clothes.
  
  Use of Untreated Water (not infused with SG Gas) for Washing – Clothes have remaining pungent body odor smell due to bacteria buildup in the fabric which becomes more evident when the person wearing the clothes begins to sweat from working or exercising. Soap residue on dry clothes become evident when resaturated with water.